- BrainDos™ formulation increases the anti-inflammatory activity by almost 2-fold after 4h
- Il-6 pro-inflammatory cytokine over 80% reduced with BrainDos™ technology
EMMETTEN, Switzerland, October 09, 2024 / Biotech Newswire / -- MetP Pharma AG, leveraging the proprietary nose-to-brain drug delivery technology BrainDos™ in internal and external product development and manufacturing programs, today announced preclinical data showing that tirzepatide in a BrainDos™ formulation reduced pro-inflammatory cytokine Il-6 by more than 80% and 2-fold stronger than with aqueous tirzepatide solution. The full effect of the IL-6 reduction with tirzepatide/BrainDos™ formulation was reached after 4 hours.
MetP Pharma investigated the anti-inflammatory effect of tirzepatide in SH-SY5Y neuroblastoma cell line. The cells were first treated with lipopolysaccharide (LPS) to induce inflammation. After 24 hours exposure to LPS, the cells were treated either with tirzepatide in a BrainDos™ formulation or aqueous tirzepatide solution (phosphate-buffered saline). The aqueous tirzepatide solution was similar to Lilly’s Mounjaro® only slightly differing in the salt composition. The anti-inflammatory effect was determined by measuring interleukine IL-6 reduction.
The tirzepatide/BrainDos™ formulation reduced IL-6 levels after 4 hours treatment by 87.9% versus a 44.6% reduction with tirzepatide solution. The anti-inflammatory effect of tirzepatide/BrainDos™ formulation was nearly complete after 4 hours treatment while with tirzepatide solution the effect was more pronounced after 24 hours treatment (44.6% versus 58.6%). The data demonstrate that the BrainDos™ formulation increases the anti-inflammatory activity by almost 2-fold at 4 hours, and 1.5- fold at 24 hours where it also shows a lasting effect exceeding the effect of the tirzepatide solution. The tirzepatide concentration in both the BrainDos™ formulation and the aqueous solution was 10nM.
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IL-6 response after 4 h drug treatment
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IL-6 response after 24 h drug treatment
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For high resolution please click the images.
Tirzepatide is a synthetic, dual GIP/GLP-1 receptor agonist that mimics the two hormones naturally produced by the gut and the brain. It is believed that their effect on the central nervous system reduces the feeling of hunger. Due to their charge, these peptide molecules do not pass the blood-brain barrier to a sufficient extent or not at all. BrainDos™ formulation is a potent nose-to-brain drug delivery technology developed to bypasses the blood-brain barrier.
Dr. Claudia Mattern, Chief Scientific Officer at MetP Pharma says: “BrainDos™ formulated tirzepatide is a potent anti-inflammatory agent outperforming the aqueous tirzepatide solution in reducing IL-6 levels. We believe that the BrainDos™ technology has the potential to increase the anti-inflammatory effect of weight-loss products such as Wegovy® and Mounjaro®.”
Recently, MetP Pharma announced preclinical results showing that GLP-1 receptor agonist semaglutide formulated with the BrainDos™ technology significantly increases drug delivery into cells (1). Chronic brain inflammation is involved in the etiopathology of many neurodegenerative diseases (2) and innovative formulations such as with BrainDos™ technology could have a broad clinical potential far beyond managing obesity.
About BrainDos™
BrainDos™ is an intranasal oil-based drug delivery system developed to bypasses the blood-brain barrier (BBB) and allowing precise drug administration. BrainDos™ consists of a thixotropic oleogel, Silcum®, enclosed in a mono-dose applicator (Lecticula®). The advanced gel system incorporates the active pharmaceutical ingredient (API) within a lipid-based matrix and exhibits distinctive intranasal drug retention and mucosal adhesion properties thus allowing optimal drug release and exposure to the nasal mucosa. Silcum® facilitates the direct entry of drugs to the brain via the olfactory and trigeminal pathways as well as the topical and systemic delivery via the different tissue types in the nasal cavity. Lecticula® is a fingernail-sized mono-dose container ensuring easy application and providing high dosing accuracy (± 3%). Its supply chain is secure, there is no risk of shortage and recycling is easy. Lecticula® was among the finalists for the 2016 CPhI Pharma Award in the category "Excellence in Pharma: Drug Delivery Devices". The filling of the single-dose containers at laboratory, pilot and industrial scale are carried out in specially designed filling machines.
About MetP Pharma AG
MetP® Pharma AG is an independent, specialized pharmaceutical R&D and product development company based in Switzerland. The company has developed BrainDos™, an advanced and comprehensively patented nose-to-brain drug delivery system allowing to bypass the blood-brain barrier. The brain targeting effect of BrainDos™ has been demonstrated through various proof-of-concept studies. BrainDos™ is a fully developed and flexible technology platform that is significantly reducing the risk of pharmaceutical product development. The company’s internal product development program includes intranasal products for the indications concussion, insomnia, attention deficit hyperactivity disorder (ADHD), multiple sclerosis (MS) and hypogonadism.
MetP® also provides pharmaceutical development services based on its proprietary and worldwide patented intranasal BrainDos™ delivery technology. The company’s research has been published in 65 peer-reviewed journals and a robust intellectual property portfolio exist in many countries until 2037/2039.
Contact
MetP Pharma AG
Dr. Claudia Mattern
CSO
+41-41-618 30 30
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References
(1) Glucagon-like peptide-1 (GLP-1) Receptor Agonist Semaglutide in an Oleogel Formulation Using the BrainDos™ Technology Significantly Increases Drug Delivery into Cells (Link)
(2) Diz-Chaves Y, Mastoor Z, Spuch C, González-Matías LC, Mallo F. Anti-Inflammatory Effects of GLP-1 Receptor Activation in the Brain in Neurodegenerative Diseases. Int J Mol Sci. 2022 Aug 24;23(17):9583.
Keywords: tirzepatide; Interleukin-6; Glucagon-Like Peptide-1 Receptor Agonists; Anti-Inflammatory Agents; Brain; Wegovy; Ozempic; oleogels; Obesity; Drug Delivery Systems; Drug Compounding; Organic Chemicals; Drug Evaluation, Preclinical; Switzerland; BrainDos™; Dual GIP/GLP-1 receptor agonist; Mounjaro®; Preclinical study; Anti-inflammatory activity; Il-6 reduction; SH-SY5Y neuroblastoma cell line; Cytokine IL-6; MetP Pharma AG; Dr. Claudia Mattern; BrainDos™ formulation; Nose-to-brain drug delivery; Blood-brain barrier; Thixotropic oleogel; GLP-1 receptor agonist; Neurodegenerative diseases; Chronic brain inflammation; Intranasal drug delivery; Obesity management; Wegovy®; Drug retention; Drug release; Olfactory pathways; Trigeminal pathways; Mono-dose container
Source: Biotech Newswire